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alkaline phosphatase substrate kit  (Vector Laboratories)


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    Vector Laboratories alkaline phosphatase substrate kit
    Alkaline <t>phosphatase</t> staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.
    Alkaline Phosphatase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SK-5200/VECTOR+Black+Alkaline+Phosphatase+(AP)+Substrate+Kit/bio_rxiv__2025__11__22__689923-47-8-7
    Average 95 stars, based on 411 article reviews
    alkaline phosphatase substrate kit - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state"

    Article Title: Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state

    Journal: bioRxiv

    doi: 10.1101/2025.11.22.689923

    Alkaline phosphatase staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.
    Figure Legend Snippet: Alkaline phosphatase staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.

    Techniques Used: Staining

    (A–D) Flattened sESC EOS-OCT4-GFP cultures transferred into HENSM medium initially underwent substantial cell death, but by passages 3–5, compact EGFP-positive dome-shaped colonies emerged. (E–H) Dome-shaped colonies were independently generated from both primed lines (sESa and sESb), retained alkaline phosphatase staining, and expressed OCT4, SOX2, and NANOG. (I-L) Upon exposure to serum-containing differentiation medium, colonies flattened, lost ALP activity, and produced cells expressing markers of all three germ layers.
    Figure Legend Snippet: (A–D) Flattened sESC EOS-OCT4-GFP cultures transferred into HENSM medium initially underwent substantial cell death, but by passages 3–5, compact EGFP-positive dome-shaped colonies emerged. (E–H) Dome-shaped colonies were independently generated from both primed lines (sESa and sESb), retained alkaline phosphatase staining, and expressed OCT4, SOX2, and NANOG. (I-L) Upon exposure to serum-containing differentiation medium, colonies flattened, lost ALP activity, and produced cells expressing markers of all three germ layers.

    Techniques Used: Generated, Staining, Activity Assay, Produced, Expressing

    A. Alkaline phosphatase staining of colonies grown in different naïve conditions. B. GFP-fluorescence intensity in Oct4-GFP colonies grown under different conditions. C. Karyotype of naïve like cells.
    Figure Legend Snippet: A. Alkaline phosphatase staining of colonies grown in different naïve conditions. B. GFP-fluorescence intensity in Oct4-GFP colonies grown under different conditions. C. Karyotype of naïve like cells.

    Techniques Used: Staining, Fluorescence

    Related Articles

    Saline:

    Article Title: Counterregulatory roles of GLI2 and GLI3 in osteogenic differentiation via Gli1 expression.
    Article Snippet: .. The cells were then fixed in 3% paraformaldehyde for 15 min at room temperature, washed with phosphate-buffered saline and stained with Black Substrate Kit Alkaline Phosphatase (Vector Laboratories) for 30 min at room temperature. .. The stained cells were washed with 100 mM Tris-HCl (pH 9.5) for 5 min and observed using an all-in-one-type fluorescence microscope EVOS M5000 (Thermo Fisher Scientific) using a 10×/0.40 objective lens.

    Article Title: Skeletal ciliopathy variants of the dynein-2 DYNC2LI1 subunit impair osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: .. The cells were fixed in 3% paraformaldehyde for 15 min at room temperature, washed with phosphatebuffered saline, and stained with Black Substrate Kit Alkaline Phosphatase (Vector Laboratories) for 30 min at room temperature. .. The stained cells were washed with 100 mM Tris-HCl (pH 9.5) for 5 min, and observed using an all-in-one-type fluorescence microscope EVOS M5000 (Thermo Fisher Scientific) using a 4×/0.13 NA objective lens.

    Staining:

    Article Title: Counterregulatory roles of GLI2 and GLI3 in osteogenic differentiation via Gli1 expression.
    Article Snippet: .. The cells were then fixed in 3% paraformaldehyde for 15 min at room temperature, washed with phosphate-buffered saline and stained with Black Substrate Kit Alkaline Phosphatase (Vector Laboratories) for 30 min at room temperature. .. The stained cells were washed with 100 mM Tris-HCl (pH 9.5) for 5 min and observed using an all-in-one-type fluorescence microscope EVOS M5000 (Thermo Fisher Scientific) using a 10×/0.40 objective lens.

    Article Title: Heat-treated and/or lysozyme-treated Enterococcus faecalis (FK-23) improves the progression of renal disease in a unilateral ischemia-reperfusion injury rat model
    Article Snippet: .. An alkaline phosphatase substrate kit (Vector Laboratories, Inc., Newark, CA) was used, and nuclear staining was performed with hematoxylin. ..

    Article Title: Heat-treated and/or lysozyme-treated Enterococcus faecalis (FK-23) improves the progression of renal disease in a unilateral ischemia-reperfusion injury rat model.
    Article Snippet: .. An alkaline phosphatase substrate kit (Vector Laborato‐ ries, Inc., Newark, CA) was used, and nuclear staining was per‐ formed with hematoxylin. ..

    Article Title: Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state
    Article Snippet: .. Staining for ALP activity was performed using Vector Alkaline Phosphatase Substrate kit as per manufacturer instructions (Vector labs). ..

    Article Title: Skeletal ciliopathy variants of the dynein-2 DYNC2LI1 subunit impair osteogenic differentiation of mesenchymal stem cells.
    Article Snippet: .. The cells were fixed in 3% paraformaldehyde for 15 min at room temperature, washed with phosphatebuffered saline, and stained with Black Substrate Kit Alkaline Phosphatase (Vector Laboratories) for 30 min at room temperature. .. The stained cells were washed with 100 mM Tris-HCl (pH 9.5) for 5 min, and observed using an all-in-one-type fluorescence microscope EVOS M5000 (Thermo Fisher Scientific) using a 4×/0.13 NA objective lens.

    Article Title: Inhibition of cytosolic DNA sensing and transposon activity safeguards pluripotency
    Article Snippet: .. On the last day, ESCs were fixed with 4% PFA and stained with an Alkaline phosphatase kit (Vector, USA) according to the manufacturer’s protocol. .. On the last day, ESCs were fixed with 4% PFA and stained with an Alkaline phosphatase kit (Vector, USA) according to the manufacturer’s protocol.

    Blocking Assay:

    Article Title: Modeling Heartland virus disease in mice and therapeutic intervention with 4'-fluorouridine.
    Article Snippet: Heartland virus (HRTV) is an emerging tick-borne bandavirus that causes a febrile illness of varying severity in humans, with cases reported in eastern and midwestern regions of the United States.. No vaccines or approved therapies are available to prevent or treat HRTV disease.. Here, we describe the genetic changes, natural history of disease, and pathogenesis of a mouse-adapted HRTV (MA-HRTV) that is uniformly lethal in 7to 8-week-old AG129 mice at low challenge doses.

    Incubation:

    Article Title: Modeling Heartland virus disease in mice and therapeutic intervention with 4'-fluorouridine.
    Article Snippet: Heartland virus (HRTV) is an emerging tick-borne bandavirus that causes a febrile illness of varying severity in humans, with cases reported in eastern and midwestern regions of the United States.. No vaccines or approved therapies are available to prevent or treat HRTV disease.. Here, we describe the genetic changes, natural history of disease, and pathogenesis of a mouse-adapted HRTV (MA-HRTV) that is uniformly lethal in 7to 8-week-old AG129 mice at low challenge doses.

    Activity Assay:

    Article Title: Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state
    Article Snippet: .. Staining for ALP activity was performed using Vector Alkaline Phosphatase Substrate kit as per manufacturer instructions (Vector labs). ..



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    alkaline phosphatase substrate kit  (Vector Laboratories)
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    Alkaline <t>phosphatase</t> staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.
    Alkaline Phosphatase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SK-5200/VECTOR+Black+Alkaline+Phosphatase+(AP)+Substrate+Kit/bio_rxiv__2025__11__22__689923-47-8-7
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    black substrate kit alkaline phosphatase  (Vector Laboratories)
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    Alkaline <t>phosphatase</t> staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.
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    a) PCA of all genes in the indicated ESC samples, showing that IRF3 GOF samples cluster separately from all other conditions along PC1. RNA-seq data are from 4 replicates from independent experiments. b) Volcano plot of differentially expressed genes between IRF3 GOF versus BFP-only transfected ESCs. Blue points are significantly differentially expressed between the conditions (FDR ≤ 0.01). A set of pluripotency genes are indicated in purple, and the majority are down-regulated in IRF3 GOF samples. Selected IFN-I genes are shown in red (triangle for IRF3 indicates −log 10 FDR >25). c) The top 15 biological processes (BP) enriched in GO analysis of significantly downregulated genes in IRF3 GOF over BFP-only conditions (FDR < 0.05). d) Colony formation assays in ESCs after transfection of the indicated constructs and sorting. ESCs were plated at low density and cultured in the presence of Dox for 5 days before fixing and staining for alkaline <t>phosphatase.</t> Data are mean +/- s.e.m for n=3 wells, representative of 3-4 experiments. P values, one-way ANOVA with Šídák’s multiple comparisons test. e) Examples and quantification of immunofluorescence experiments for ESRRB and NANOG, 36 h after transfection with BFP or IRF3 GOF and FACS. ESCs were plated onto Matrigel immediately after sorting and fixed 1h later. P values, Welch’s t-test. f) As in e, but staining for OCT4 and SOX2. g) Morphology and alkaline phosphatase staining of parental KH2 ESCs or stable IRF3 GOF ESCs (clone B5, E7) showing the latter begin to lose undifferentiated morphology by 72 h after Dox addition. Data are representative of 3 clones and 2 experiments. h) Quantification of ESC differentiation status in g), according to AP staining. Colonies were scored according to the criteria of undifferentiated: >80% AP-positive, mixed: 30-80% AP-positive, differentiated: <30% AP-positive. Data shown are from 2 experiments. P values, Chi-squared test with Bonferroni correction for multiple comparisons. i) Colony formation assay in KH2 parental ESCs or IRF3 GOF ESCs (clone E7) 5 days after plating at low density in the presence or absence of Dox. All data in figure are representative of at least 2 independent experiments unless otherwise indicated.
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    a) PCA of all genes in the indicated ESC samples, showing that IRF3 GOF samples cluster separately from all other conditions along PC1. RNA-seq data are from 4 replicates from independent experiments. b) Volcano plot of differentially expressed genes between IRF3 GOF versus BFP-only transfected ESCs. Blue points are significantly differentially expressed between the conditions (FDR ≤ 0.01). A set of pluripotency genes are indicated in purple, and the majority are down-regulated in IRF3 GOF samples. Selected IFN-I genes are shown in red (triangle for IRF3 indicates −log 10 FDR >25). c) The top 15 biological processes (BP) enriched in GO analysis of significantly downregulated genes in IRF3 GOF over BFP-only conditions (FDR < 0.05). d) Colony formation assays in ESCs after transfection of the indicated constructs and sorting. ESCs were plated at low density and cultured in the presence of Dox for 5 days before fixing and staining for alkaline <t>phosphatase.</t> Data are mean +/- s.e.m for n=3 wells, representative of 3-4 experiments. P values, one-way ANOVA with Šídák’s multiple comparisons test. e) Examples and quantification of immunofluorescence experiments for ESRRB and NANOG, 36 h after transfection with BFP or IRF3 GOF and FACS. ESCs were plated onto Matrigel immediately after sorting and fixed 1h later. P values, Welch’s t-test. f) As in e, but staining for OCT4 and SOX2. g) Morphology and alkaline phosphatase staining of parental KH2 ESCs or stable IRF3 GOF ESCs (clone B5, E7) showing the latter begin to lose undifferentiated morphology by 72 h after Dox addition. Data are representative of 3 clones and 2 experiments. h) Quantification of ESC differentiation status in g), according to AP staining. Colonies were scored according to the criteria of undifferentiated: >80% AP-positive, mixed: 30-80% AP-positive, differentiated: <30% AP-positive. Data shown are from 2 experiments. P values, Chi-squared test with Bonferroni correction for multiple comparisons. i) Colony formation assay in KH2 parental ESCs or IRF3 GOF ESCs (clone E7) 5 days after plating at low density in the presence or absence of Dox. All data in figure are representative of at least 2 independent experiments unless otherwise indicated.
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    Image Search Results


    Alkaline phosphatase staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.

    Journal: bioRxiv

    Article Title: Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state

    doi: 10.1101/2025.11.22.689923

    Figure Lengend Snippet: Alkaline phosphatase staining of colonies grown in different matrices. B. Withdrawal of WNT inhibitors from culture condition led to differentiation, evidenced by the loss of colony definition and the emergence of morphologically distinct differentiated cells.

    Article Snippet: Staining for ALP activity was performed using Vector Alkaline Phosphatase Substrate kit as per manufacturer instructions (Vector labs).

    Techniques: Staining

    (A–D) Flattened sESC EOS-OCT4-GFP cultures transferred into HENSM medium initially underwent substantial cell death, but by passages 3–5, compact EGFP-positive dome-shaped colonies emerged. (E–H) Dome-shaped colonies were independently generated from both primed lines (sESa and sESb), retained alkaline phosphatase staining, and expressed OCT4, SOX2, and NANOG. (I-L) Upon exposure to serum-containing differentiation medium, colonies flattened, lost ALP activity, and produced cells expressing markers of all three germ layers.

    Journal: bioRxiv

    Article Title: Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state

    doi: 10.1101/2025.11.22.689923

    Figure Lengend Snippet: (A–D) Flattened sESC EOS-OCT4-GFP cultures transferred into HENSM medium initially underwent substantial cell death, but by passages 3–5, compact EGFP-positive dome-shaped colonies emerged. (E–H) Dome-shaped colonies were independently generated from both primed lines (sESa and sESb), retained alkaline phosphatase staining, and expressed OCT4, SOX2, and NANOG. (I-L) Upon exposure to serum-containing differentiation medium, colonies flattened, lost ALP activity, and produced cells expressing markers of all three germ layers.

    Article Snippet: Staining for ALP activity was performed using Vector Alkaline Phosphatase Substrate kit as per manufacturer instructions (Vector labs).

    Techniques: Generated, Staining, Activity Assay, Produced, Expressing

    A. Alkaline phosphatase staining of colonies grown in different naïve conditions. B. GFP-fluorescence intensity in Oct4-GFP colonies grown under different conditions. C. Karyotype of naïve like cells.

    Journal: bioRxiv

    Article Title: Derivation of primed sheep embryonic stem cells and conversion to an intermediate naïve-like state

    doi: 10.1101/2025.11.22.689923

    Figure Lengend Snippet: A. Alkaline phosphatase staining of colonies grown in different naïve conditions. B. GFP-fluorescence intensity in Oct4-GFP colonies grown under different conditions. C. Karyotype of naïve like cells.

    Article Snippet: Staining for ALP activity was performed using Vector Alkaline Phosphatase Substrate kit as per manufacturer instructions (Vector labs).

    Techniques: Staining, Fluorescence

    a) PCA of all genes in the indicated ESC samples, showing that IRF3 GOF samples cluster separately from all other conditions along PC1. RNA-seq data are from 4 replicates from independent experiments. b) Volcano plot of differentially expressed genes between IRF3 GOF versus BFP-only transfected ESCs. Blue points are significantly differentially expressed between the conditions (FDR ≤ 0.01). A set of pluripotency genes are indicated in purple, and the majority are down-regulated in IRF3 GOF samples. Selected IFN-I genes are shown in red (triangle for IRF3 indicates −log 10 FDR >25). c) The top 15 biological processes (BP) enriched in GO analysis of significantly downregulated genes in IRF3 GOF over BFP-only conditions (FDR < 0.05). d) Colony formation assays in ESCs after transfection of the indicated constructs and sorting. ESCs were plated at low density and cultured in the presence of Dox for 5 days before fixing and staining for alkaline phosphatase. Data are mean +/- s.e.m for n=3 wells, representative of 3-4 experiments. P values, one-way ANOVA with Šídák’s multiple comparisons test. e) Examples and quantification of immunofluorescence experiments for ESRRB and NANOG, 36 h after transfection with BFP or IRF3 GOF and FACS. ESCs were plated onto Matrigel immediately after sorting and fixed 1h later. P values, Welch’s t-test. f) As in e, but staining for OCT4 and SOX2. g) Morphology and alkaline phosphatase staining of parental KH2 ESCs or stable IRF3 GOF ESCs (clone B5, E7) showing the latter begin to lose undifferentiated morphology by 72 h after Dox addition. Data are representative of 3 clones and 2 experiments. h) Quantification of ESC differentiation status in g), according to AP staining. Colonies were scored according to the criteria of undifferentiated: >80% AP-positive, mixed: 30-80% AP-positive, differentiated: <30% AP-positive. Data shown are from 2 experiments. P values, Chi-squared test with Bonferroni correction for multiple comparisons. i) Colony formation assay in KH2 parental ESCs or IRF3 GOF ESCs (clone E7) 5 days after plating at low density in the presence or absence of Dox. All data in figure are representative of at least 2 independent experiments unless otherwise indicated.

    Journal: bioRxiv

    Article Title: Inhibition of cytosolic DNA sensing and transposon activity safeguards pluripotency

    doi: 10.1101/2025.03.26.645264

    Figure Lengend Snippet: a) PCA of all genes in the indicated ESC samples, showing that IRF3 GOF samples cluster separately from all other conditions along PC1. RNA-seq data are from 4 replicates from independent experiments. b) Volcano plot of differentially expressed genes between IRF3 GOF versus BFP-only transfected ESCs. Blue points are significantly differentially expressed between the conditions (FDR ≤ 0.01). A set of pluripotency genes are indicated in purple, and the majority are down-regulated in IRF3 GOF samples. Selected IFN-I genes are shown in red (triangle for IRF3 indicates −log 10 FDR >25). c) The top 15 biological processes (BP) enriched in GO analysis of significantly downregulated genes in IRF3 GOF over BFP-only conditions (FDR < 0.05). d) Colony formation assays in ESCs after transfection of the indicated constructs and sorting. ESCs were plated at low density and cultured in the presence of Dox for 5 days before fixing and staining for alkaline phosphatase. Data are mean +/- s.e.m for n=3 wells, representative of 3-4 experiments. P values, one-way ANOVA with Šídák’s multiple comparisons test. e) Examples and quantification of immunofluorescence experiments for ESRRB and NANOG, 36 h after transfection with BFP or IRF3 GOF and FACS. ESCs were plated onto Matrigel immediately after sorting and fixed 1h later. P values, Welch’s t-test. f) As in e, but staining for OCT4 and SOX2. g) Morphology and alkaline phosphatase staining of parental KH2 ESCs or stable IRF3 GOF ESCs (clone B5, E7) showing the latter begin to lose undifferentiated morphology by 72 h after Dox addition. Data are representative of 3 clones and 2 experiments. h) Quantification of ESC differentiation status in g), according to AP staining. Colonies were scored according to the criteria of undifferentiated: >80% AP-positive, mixed: 30-80% AP-positive, differentiated: <30% AP-positive. Data shown are from 2 experiments. P values, Chi-squared test with Bonferroni correction for multiple comparisons. i) Colony formation assay in KH2 parental ESCs or IRF3 GOF ESCs (clone E7) 5 days after plating at low density in the presence or absence of Dox. All data in figure are representative of at least 2 independent experiments unless otherwise indicated.

    Article Snippet: On the last day, ESCs were fixed with 4% PFA and stained with an Alkaline phosphatase kit (Vector, USA) according to the manufacturer’s protocol.

    Techniques: RNA Sequencing, Transfection, Construct, Cell Culture, Staining, Immunofluorescence, Clone Assay, Colony Assay